5AKO
The complex of Tse2 and Tsi2 from Pseudomonas aeruginosa
Summary for 5AKO
| Entry DOI | 10.2210/pdb5ako/pdb |
| Descriptor | TSI2, TSE2 (3 entities in total) |
| Functional Keywords | unknown function, t6ss, immunity protein, toxin |
| Biological source | PSEUDOMONAS AERUGINOSA More |
| Total number of polymer chains | 4 |
| Total formula weight | 57015.92 |
| Authors | Robb, C.S.,Nano, F.E.,Boraston, A.B. (deposition date: 2015-03-04, release date: 2015-12-23, Last modification date: 2024-01-10) |
| Primary citation | Robb, C.S.,Robb, M.,Nano, F.E.,Boraston, A.B. The Structure of the Toxin and Type Six Secretion System Substrate Tse2 in Complex with its Immunity Protein. Structure, 24:277-, 2016 Cited by PubMed Abstract: Tse2 is a cytoactive toxin secreted by a type six secretion apparatus of Pseudomonas aeruginosa. The Tse2 toxin naturally attacks a target in the cytoplasm of bacterial cells, and can cause toxicity if artificially introduced into eukaryotic cells. The X-ray crystal structure of the complex of Tse2 and its cognate immunity protein Tsi2 revealed a heterotetrameric structure with an extensive binding interface. Structural identity was found between Tse2 and NAD-dependent enzymes, especially ADP-ribosylating toxins, which facilitated the identification of the Tse2 active site and revealed it to be occluded upon binding the inhibitor Tsi2. The structural identity shared with NAD-dependent enzymes, including conserved catalytic residues, suggests that the mechanism of Tse2 toxicity may be NAD dependent. PubMed: 26749446DOI: 10.1016/J.STR.2015.11.012 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
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