5AKO
The complex of Tse2 and Tsi2 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 113 |
| Detector technology | PIXEL |
| Collection date | 2013-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.725, 103.138, 114.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.830 - 2.400 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vpv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.543 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.840 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.730 |
| Number of reflections | 17725 | |
| <I/σ(I)> | 13.4 | 2.3 |
| Completeness [%] | 97.6 | 99.1 |
| Redundancy | 4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 20 MM TRIS PH 8.5, 0.2 M NACL, 22% PEG 4K |
