5AGD
An inactive (D125N) variant of the catalytic domain, BcGH76, of Bacillus circulans Aman6 in complex with alpha-1,6-mannopentaose
Summary for 5AGD
Entry DOI | 10.2210/pdb5agd/pdb |
Related | 4D4A 4D4B 4D4C 4D4D |
Descriptor | ALPHA-1,6-MANNANASE, alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose, alpha-D-mannopyranose, ... (4 entities in total) |
Functional Keywords | hydrolase, alpha-mannanase, mannanase, glycoside hydrolase, gh76, cazy, mannan, enzyme-carbohydrate interaction, glycosidase inhibition, quantum mechanics, transition state |
Biological source | BACILLUS CIRCULANS |
Total number of polymer chains | 2 |
Total formula weight | 83877.50 |
Authors | Thompson, A.J.,Speciale, G.,Iglesias-Fernandez, J.,Hakki, Z.,Belz, T.,Cartmell, A.,Spears, R.J.,Chandler, E.,Temple, M.J.,Stepper, J.,Gilbert, H.J.,Rovira, C.,Williams, S.J.,Davies, G.J. (deposition date: 2015-01-29, release date: 2015-03-25, Last modification date: 2024-05-01) |
Primary citation | Thompson, A.J.,Speciale, G.,Iglesias-Fernandez, J.,Hakki, Z.,Belz, T.,Cartmell, A.,Spears, R.J.,Chandler, E.,Temple, M.J.,Stepper, J.,Gilbert, H.J.,Rovira, C.,Williams, S.J.,Davies, G.J. Evidence for a Boat Conformation at the Transition State of Gh76 Alpha-1,6-Mannanases- Key Enzymes in Bacterial and Fungal Mannoprotein Metabolism Angew.Chem.Int.Ed.Engl., 54:5378-, 2015 Cited by PubMed: 25772148DOI: 10.1002/ANIE.201410502 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.2 Å) |
Structure validation
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