5AGD

An inactive (D125N) variant of the catalytic domain, BcGH76, of Bacillus circulans Aman6 in complex with alpha-1,6-mannopentaose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ALPHA-1,6-MANNANASE	polymer	362	40929.9	2	UniProt (Q9Z4P9) Pfam (PF03663) In PDB	BACILLUS CIRCULANS
2	C, D	alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose	branched		828.7	2	In PDB GlyTouCan (G20043ZO)
3	A, B	alpha-D-mannopyranose	non-polymer		180.2	2	Chemie (MAN)
4		water	water		18.0	1032	Chemie (HOH)

Sequence modifications

A, B: 35 - 375 (UniProt: Q9Z4P9)

PDB	External Database	Details
Met 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Ser 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
Ser 24	-	expression tag
Ser 25	-	expression tag
Gly 26	-	expression tag
Leu 27	-	expression tag
Glu 28	-	expression tag
Val 29	-	expression tag
Leu 30	-	expression tag
Phe 31	-	expression tag
Gln 32	-	expression tag
Gly 33	-	expression tag
Pro 34	-	expression tag
Asn 125	Asp 125	engineered mutation
Gln 341	Arg 341	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81859.8
Branched	Number of molecules	2
	Total formula weight	1657.4
Non-Polymers*	Number of molecules	2
	Total formula weight	360.3
All*	Total formula weight	83877.5

*Water molecules are not included.