5AEE

A bacterial protein structure in glycoside hydrolase family 31

Summary for 5AEE

• Entry DOI: 10.2210/pdb5aee/pdb
• Related: 5AED 5AEG
• Descriptor: ALPHA-GLUCOSIDASE YIHQ, CALCIUM ION, 4-nitrophenyl alpha-D-6-sulfoquinovoside, ... (6 entities in total)
• Functional Keywords: hydrolase, gh31, alpha-sulfoquinovosidase
• Biological source: ESCHERICHIA COLI
• Total number of polymer chains: 2
• Total formula weight: 157850.32

Authors

Jin, Y.,Speciale, G.,Davies, G.J.,Williams, S.J.,Goddard-Borger, E.D. (deposition date: 2015-08-28, release date: 2016-02-10, Last modification date: 2024-01-10)

Primary citation

Speciale, G.,Jin, Y.,Davies, G.J.,Williams, S.J.,Goddard-Borger, E.D.
Yihq is a Sulfoquinovosidase that Cleaves Sulfoquinovosyl Diacylglyceride Sulfolipids.
Nat.Chem.Biol., 12:215-, 2016

PubMed Abstract: Sulfoquinovose is produced by photosynthetic organisms at a rate of 10(10) tons per annum and is degraded by bacteria as a source of carbon and sulfur. We have identified Escherichia coli YihQ as the first dedicated sulfoquinovosidase and the gateway enzyme to sulfoglycolytic pathways. Structural and mutagenesis studies unveiled the sequence signatures for binding the distinguishing sulfonate residue and revealed that sulfoquinovoside degradation is widespread across the tree of life.

PubMed: 26878550
DOI: 10.1038/NCHEMBIO.2023

Experimental method

X-RAY DIFFRACTION (1.85 Å)