5AEE

A bacterial protein structure in glycoside hydrolase family 31

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ALPHA-GLUCOSIDASE YIHQ	polymer	686	78422.9	2	UniProt (P32138) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365) In PDB	ESCHERICHIA COLI	ALPHA-SULFOQUINOVOSIDASE
2	A, B	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
3	A, B	4-nitrophenyl alpha-D-6-sulfoquinovoside	non-polymer		365.3	2	Chemie (NSQ)
4	B	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
5	B	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6		water	water		18.0	602	Chemie (HOH)

Sequence modifications

A, B: 1 - 678 (UniProt: P32138)

PDB	External Database	Details
Leu 679	-	expression tag
Glu 680	-	expression tag
His 681	-	expression tag
His 682	-	expression tag
His 683	-	expression tag
His 684	-	expression tag
His 685	-	expression tag
His 686	-	expression tag
Asn 472	Asp 472	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	156845.8
Non-Polymers*	Number of molecules	7
	Total formula weight	1004.5
All*	Total formula weight	157850.3

*Water molecules are not included.