5A77

Crystal structure of the homing endonuclease I-CvuI in complex with I- CreI target (C1221) in the presence of 2 mM Mg revealing DNA cleaved

Summary for 5A77

• Entry DOI: 10.2210/pdb5a77/pdb
• Related: 5A72 5A73 5A74 5A75 5A78
• Descriptor: DNA ENDONUCLEASE I-CVUI, 14MER DNA, 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*DCP)-3', 10MER DNA, 5'-D(*GP*AP*CP*GP*TP*TP*TP*TP* GP*AP*DGP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3', ... (5 entities in total)
• Functional Keywords: hydrolase-dna complex, gene targeting, genetics, protein-dna interaction, homing endonucleases, hydrolase/dna
• Biological source: CHLORELLA VULGARIS; More
• Cellular location: Plastid, chloroplast: P56347
• Total number of polymer chains: 6
• Total formula weight: 53281.31

Authors

Molina, R.,Redondo, P.,LopezMendez, B.,Villate, M.,Merino, N.,Blanco, F.J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. (deposition date: 2015-07-03, release date: 2015-09-23, Last modification date: 2024-01-10)

• Primary citation: Molina, R.,Redondo, P.,Lopez-Mendez, B.,Villate, M.,Merino, N.,Blanco, F.J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G.; Crystal Structure of the Homing Endonuclease I-Cvui Provides a New Template for Genome Modification; J.Biol.Chem., 290:28727-, 2015; PubMed: 26363068; DOI: 10.1074/JBC.M115.678342

Experimental method

X-RAY DIFFRACTION (2.5 Å)