5A5W

Crystal structure of Salmonella enterica HisA D7N D176A with ProFAR

Summary for 5A5W

Descriptor, [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate (3 entities in total)
Functional Keywordsisomerase, histidine biosynthesis, profar
Biological sourceSALMONELLA ENTERICA
Cellular locationCytoplasm P10372
Total number of polymer chains1
Total molecular weight27664.27
Authors
Soderholm, A.,Guo, X.,Newton, M.S.,Evans, G.B.,Nasvall, J.,Patrick, W.M.,Selmer, M. (deposition date: 2015-06-23, release date: 2015-09-02, Last modification date: 2015-10-21)
Primary citation
Soderholm, A.,Guo, X.,Newton, M.S.,Evans, G.B.,Nasvall, J.,Patrick, W.M.,Selmer, M.
Two-Step Ligand Binding in a Beta/Alpha8 Barrel Enzyme -Substrate-Bound Structures Shed New Light on the Catalytic Cycle of Hisa
J.Biol.Chem., 290:24657-, 2015
PubMed: 26294764 (PDB entries with the same primary citation)
DOI: 10.1074/JBC.M115.678086
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.6 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.200300.5%0MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5a5w
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Molmil generated image of 5a5w
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Molmil generated image of 5a5w
rotated about y axis by 90°