5Y1Z
Crystal structure of ZMYND8 PHD-BROMO-PWWP tandem in complex with Drebrin ADF-H domain
Summary for 5Y1Z
| Entry DOI | 10.2210/pdb5y1z/pdb |
| Descriptor | Drebrin, Protein kinase C-binding protein 1, SULFATE ION, ... (6 entities in total) |
| Functional Keywords | transcription, nuclear protein, protein binding, actin binding |
| Biological source | Homo sapiens (Human) More |
| Cellular location | Cytoplasm : Q16643 Nucleus : Q9ULU4 |
| Total number of polymer chains | 4 |
| Total formula weight | 105646.31 |
| Authors | |
| Primary citation | Yao, N.,Li, J.,Liu, H.,Wan, J.,Liu, W.,Zhang, M. The Structure of the ZMYND8/Drebrin Complex Suggests a Cytoplasmic Sequestering Mechanism of ZMYND8 by Drebrin Structure, 25:1657-1666.e3, 2017 Cited by PubMed Abstract: Malfunctions of the actin binding protein Drebrin have been implicated in various human diseases such as Alzheimer's disease, cognitive impairments, cancer, and digestive disorders, though with poorly understood mechanisms. The ADF-H domain of Drebrin does not contain actin binding and depolymerizing activity. Instead, it binds to a histone marker reader, ZMYND8. Here we present the high-resolution crystal structure of Drebrin ADF-H in complex with the ZMYND8 PHD-BROMO-PWWP tandem, elucidating the mechanistic basis governing the highly specific interaction of the two proteins. The structure reveals that the ZMYND8 PHD-BROMO-PWWP tandem forms a structural supramodule that is necessary for binding to Drebrin ADF-H. Drebrin ADF-H competes with modified histone for binding to ZMYND8. Binding of Drebrin can shuttle ZMYND8 from nucleus to cytoplasm in living cells. Taken together, our study uncovers a non-actin target binding mode for ADF-H domains, and suggests that Drebrin may regulate activities of epigenetic reader ZMYND8 via its cytoplasmic sequestration. PubMed: 28966017DOI: 10.1016/j.str.2017.08.014 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.676 Å) |
Structure validation
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