5Y1Z
Crystal structure of ZMYND8 PHD-BROMO-PWWP tandem in complex with Drebrin ADF-H domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-01-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9780 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.183, 138.095, 161.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.700 |
High resolution limit [Å] | 2.650 | 7.190 | 2.650 |
Rmerge | 0.092 | 0.036 | 0.951 |
Rmeas | 0.100 | 0.039 | 1.031 |
Rpim | 0.038 | 0.015 | 0.392 |
Number of reflections | 34299 | 1638 | |
<I/σ(I)> | 11.1 | ||
Completeness [%] | 98.9 | 98.8 | 94.5 |
Redundancy | 6.5 | 6.6 | 6.3 |
CC(1/2) | 0.999 | 0.758 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 6.0 and 23% w/v polyethylene glycol 3,350 |