5TCC

Complement Factor D inhibited with JH4

Summary for 5TCC

• Entry DOI: 10.2210/pdb5tcc/pdb
• Related: 5TCA
• Descriptor: Complement factor D, (2S)-N-(6-bromopyridin-2-yl)-3-[(1H-indazol-1-yl)acetyl]-1,3-thiazolidine-2-carboxamide (2 entities in total)
• Functional Keywords: serine protease, inhibitor, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 7
• Total formula weight: 175088.54

Authors

Stuckey, J.A. (deposition date: 2016-09-14, release date: 2016-10-19, Last modification date: 2024-10-30)

Primary citation

Yang, C.Y.,Phillips, J.G.,Stuckey, J.A.,Bai, L.,Sun, H.,Delproposto, J.,Brown, W.C.,Chinnaswamy, K.
Buried Hydrogen Bond Interactions Contribute to the High Potency of Complement Factor D Inhibitors.
ACS Med Chem Lett, 7:1092-1096, 2016

PubMed Abstract: Aberrant activation of the complement system is associated with diseases, including paroxysmal nocturnal hemoglobinuria and age-related macular degeneration. Complement factor D is the rate-limiting enzyme for activating the alternative pathway in the complement system. Recent development led to a class of potent amide containing pyrrolidine derived factor D inhibitors. Here, we used biochemical enzymatic and biolayer interferometry assays to demonstrate that the amide group improves the inhibitor potency by more than 80-fold. Our crystal structures revealed buried hydrogen bond interactions are important. Molecular orbital analysis from quantum chemistry calculations dissects the chemical groups participating in these interactions. Free energy calculation supports the differential contributions of the amide group to the binding affinities of these inhibitors. Cell-based hemolysis assay confirmed these compounds inhibit factor D mediated complement activation via the alternative pathway. Our study highlights the important interactions contributing to the high potency of factor D inhibitors reported recently.

PubMed: 27994744
DOI: 10.1021/acsmedchemlett.6b00299

Experimental method

X-RAY DIFFRACTION (3.37 Å)