5MXX

Crystal structure of human SR protein kinase 1 (SRPK1) in complex with compound 1

Summary for 5MXX

• Entry DOI: 10.2210/pdb5mxx/pdb
• Descriptor: SRPK1, 5-methyl-~{N}-[2-(4-methylpiperazin-1-yl)-5-(trifluoromethyl)phenyl]furan-2-carboxamide, 1,2-ETHANEDIOL, ... (6 entities in total)
• Functional Keywords: splicing, phosphorylation, structural genomics, structural genomics consortium, sgc
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 46826.45

Authors

Tallant, C.,Redondo, C.,Batson, J.,Toop, H.D.,Babaebi-Jadidib, R.,Savitsky, P.,Elkins, J.M.,Newman, J.A.,Burgess-Brown, N.,von Delft, F.,Arrowsmith, C.H.,Edwards, A.M.,Bountra, C.,Bates, D.O.,Morris, J.C.,Knapp, S.,Structural Genomics Consortium (SGC) (deposition date: 2017-01-25, release date: 2017-05-24, Last modification date: 2024-01-17)

Primary citation

Batson, J.,Toop, H.D.,Redondo, C.,Babaei-Jadidi, R.,Chaikuad, A.,Wearmouth, S.F.,Gibbons, B.,Allen, C.,Tallant, C.,Zhang, J.,Du, C.,Hancox, J.C.,Hawtrey, T.,Da Rocha, J.,Griffith, R.,Knapp, S.,Bates, D.O.,Morris, J.C.
Development of Potent, Selective SRPK1 Inhibitors as Potential Topical Therapeutics for Neovascular Eye Disease.
ACS Chem. Biol., 12:825-832, 2017

PubMed Abstract: Serine/arginine-protein kinase 1 (SRPK1) regulates alternative splicing of VEGF-A to pro-angiogenic isoforms and SRPK1 inhibition can restore the balance of pro/antiangiogenic isoforms to normal physiological levels. The lack of potency and selectivity of available compounds has limited development of SRPK1 inhibitors, with the control of alternative splicing by splicing factor-specific kinases yet to be translated. We present here compounds that occupy a binding pocket created by the unique helical insert of SRPK1, and trigger a backbone flip in the hinge region, that results in potent (<10 nM) and selective inhibition of SRPK1 kinase activity. Treatment with these inhibitors inhibited SRPK1 activity and phosphorylation of serine/arginine splicing factor 1 (SRSF1), resulting in alternative splicing of VEGF-A from pro-angiogenic to antiangiogenic isoforms. This property resulted in potent inhibition of blood vessel growth in models of choroidal angiogenesis in vivo. This work identifies tool compounds for splice isoform selective targeting of pro-angiogenic VEGF, which may lead to new therapeutic strategies for a diversity of diseases where dysfunctional splicing drives disease development.

PubMed: 28135068
DOI: 10.1021/acschembio.6b01048

Experimental method

X-RAY DIFFRACTION (1.75 Å)