5MXX
Crystal structure of human SR protein kinase 1 (SRPK1) in complex with compound 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 82.572, 82.572, 133.837 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.290 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.059 | 0.826 |
Rpim | 0.023 | 0.323 |
Number of reflections | 47639 | 2468 |
<I/σ(I)> | 28.4 | 3.7 |
Completeness [%] | 99.8 | 96 |
Redundancy | 14.2 | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.1 M tris pH 7.5, 0.1 M CaCl2, 20% PEG6K, 11% ethylene glycol |