5DF1

Iridoid synthase from Catharanthus roseus - ternary complex with NADP+ and geranic acid

Summary for 5DF1

• Entry DOI: 10.2210/pdb5df1/pdb
• Descriptor: Iridoid synthase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, (2E)-3,7-dimethylocta-2,6-dienoic acid, ... (6 entities in total)
• Functional Keywords: oxidoreductase, iridoid synthase, short chain dehydrogenase, nadph-dependent, catharanthus roseus
• Biological source: Catharanthus roseus (Madagascar periwinkle)
• Cellular location: Cytoplasm, cytosol : K7WDL7
• Total number of polymer chains: 2
• Total formula weight: 85051.78

Authors

Caputi, L.,Kries, H.,Stevenson, C.E.M.,Kamileen, M.O.,Sherden, N.H.,Geu-Flores, F.,Lawson, D.M.,O'Connor, S.E. (deposition date: 2015-08-26, release date: 2015-10-28, Last modification date: 2024-01-10)

Primary citation

Kries, H.,Caputi, L.,Stevenson, C.E.,Kamileen, M.O.,Sherden, N.H.,Geu-Flores, F.,Lawson, D.M.,O'Connor, S.E.
Structural determinants of reductive terpene cyclization in iridoid biosynthesis.
Nat.Chem.Biol., 12:6-8, 2016

PubMed Abstract: The carbon skeleton of ecologically and pharmacologically important iridoid monoterpenes is formed in a reductive cyclization reaction unrelated to canonical terpene cyclization. Here we report the crystal structure of the recently discovered iridoid cyclase (from Catharanthus roseus) bound to a mechanism-inspired inhibitor that illuminates substrate binding and catalytic function of the enzyme. Key features that distinguish iridoid synthase from its close homolog progesterone 5β-reductase are highlighted.

PubMed: 26551396
DOI: 10.1038/nchembio.1955

Experimental method

X-RAY DIFFRACTION (1.75 Å)