4ZWJ

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

Summary for 4ZWJ

• Entry DOI: 10.2210/pdb4zwj/pdb
• Descriptor: Chimera protein of human Rhodopsin, mouse S-arrestin, and T4 Endolysin (1 entity in total)
• Functional Keywords: gpcr, rhodopsin, visual arrestin, x-ray free electron laser, serial femtosecond crystallography, signaling protein, structural genomics, gpcr network
• Biological source: Enterobacteria phage T4; More
• Cellular location: Cell projection, cilium, photoreceptor outer segment : P20443
• Total number of polymer chains: 4
• Total formula weight: 403444.41

Authors

Kang, Y.,Zhou, X.E.,Gao, X.,He, Y.,Liu, W.,Ishchenko, A.,Barty, A.,White, T.A.,Yefanov, O.,Han, G.W.,Xu, Q.,de Waal, P.W.,Ke, J.,Tan, M.H.E.,Zhang, C.,Moeller, A.,West, G.M.,Pascal, B.,Eps, N.V.,Caro, L.N.,Vishnivetskiy, S.A.,Lee, R.J.,Suino-Powell, K.M.,Gu, X.,Pal, K.,Ma, J.,Zhi, X.,Boutet, S.,Williams, G.J.,Messerschmidt, M.,Gati, C.,Zatsepin, N.A.,Wang, D.,James, D.,Basu, S.,Roy-Chowdhury, S.,Conrad, C.,Coe, J.,Liu, H.,Lisova, S.,Kupitz, C.,Grotjohann, I.,Fromme, R.,Jiang, Y.,Tan, M.,Yang, H.,Li, J.,Wang, M.,Zheng, Z.,Li, D.,Howe, N.,Zhao, Y.,Standfuss, J.,Diederichs, K.,Dong, Y.,Potter, C.S.,Carragher, B.,Caffrey, M.,Jiang, H.,Chapman, H.N.,Spence, J.C.H.,Fromme, P.,Weierstall, U.,Ernst, O.P.,Katritch, V.,Gurevich, V.V.,Griffin, P.R.,Hubbell, W.L.,Stevens, R.C.,Cherezov, V.,Melcher, K.,Xu, H.E.,GPCR Network (GPCR) (deposition date: 2015-05-19, release date: 2015-07-29, Last modification date: 2024-11-20)

Primary citation

Kang, Y.,Zhou, X.E.,Gao, X.,He, Y.,Liu, W.,Ishchenko, A.,Barty, A.,White, T.A.,Yefanov, O.,Han, G.W.,Xu, Q.,de Waal, P.W.,Ke, J.,Tan, M.H.,Zhang, C.,Moeller, A.,West, G.M.,Pascal, B.D.,Van Eps, N.,Caro, L.N.,Vishnivetskiy, S.A.,Lee, R.J.,Suino-Powell, K.M.,Gu, X.,Pal, K.,Ma, J.,Zhi, X.,Boutet, S.,Williams, G.J.,Messerschmidt, M.,Gati, C.,Zatsepin, N.A.,Wang, D.,James, D.,Basu, S.,Roy-Chowdhury, S.,Conrad, C.E.,Coe, J.,Liu, H.,Lisova, S.,Kupitz, C.,Grotjohann, I.,Fromme, R.,Jiang, Y.,Tan, M.,Yang, H.,Li, J.,Wang, M.,Zheng, Z.,Li, D.,Howe, N.,Zhao, Y.,Standfuss, J.,Diederichs, K.,Dong, Y.,Potter, C.S.,Carragher, B.,Caffrey, M.,Jiang, H.,Chapman, H.N.,Spence, J.C.,Fromme, P.,Weierstall, U.,Ernst, O.P.,Katritch, V.,Gurevich, V.V.,Griffin, P.R.,Hubbell, W.L.,Stevens, R.C.,Cherezov, V.,Melcher, K.,Xu, H.E.
Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser.
Nature, 523:561-567, 2015

PubMed Abstract: G-protein-coupled receptors (GPCRs) signal primarily through G proteins or arrestins. Arrestin binding to GPCRs blocks G protein interaction and redirects signalling to numerous G-protein-independent pathways. Here we report the crystal structure of a constitutively active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin, determined by serial femtosecond X-ray laser crystallography. Together with extensive biochemical and mutagenesis data, the structure reveals an overall architecture of the rhodopsin-arrestin assembly in which rhodopsin uses distinct structural elements, including transmembrane helix 7 and helix 8, to recruit arrestin. Correspondingly, arrestin adopts the pre-activated conformation, with a ∼20° rotation between the amino and carboxy domains, which opens up a cleft in arrestin to accommodate a short helix formed by the second intracellular loop of rhodopsin. This structure provides a basis for understanding GPCR-mediated arrestin-biased signalling and demonstrates the power of X-ray lasers for advancing the frontiers of structural biology.

PubMed: 26200343
DOI: 10.1038/nature14656

Experimental method

X-RAY DIFFRACTION (3.302 Å)