4ZKC
The chemokine binding protein of orf virus complexed with CCL7
Summary for 4ZKC
| Entry DOI | 10.2210/pdb4zkc/pdb |
| Related | 4ZK9 4ZKB |
| Descriptor | Chemokine binding protein, C-C motif chemokine 7, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total) |
| Functional Keywords | complex, orf virus chemokine binding protein, ccl7, viral protein-cytokine complex, viral protein/cytokine |
| Biological source | Orf virus (strain NZ2) (ORFV) More |
| Total number of polymer chains | 2 |
| Total formula weight | 41188.92 |
| Authors | Knapp, K.M.,Nakatani, Y.,Krause, K.L. (deposition date: 2015-04-30, release date: 2015-07-08, Last modification date: 2024-10-23) |
| Primary citation | Counago, R.M.,Knapp, K.M.,Nakatani, Y.,Fleming, S.B.,Corbett, M.,Wise, L.M.,Mercer, A.A.,Krause, K.L. Structures of Orf Virus Chemokine Binding Protein in Complex with Host Chemokines Reveal Clues to Broad Binding Specificity. Structure, 23:1199-1213, 2015 Cited by PubMed Abstract: The chemokine binding protein (CKBP) from orf virus (ORFV) binds with high affinity to chemokines from three classes, C, CC, and CXC, making it unique among poxvirus CKBPs described to date. We present its crystal structure alone and in complex with three CC chemokines, CCL2, CCL3, and CCL7. ORFV CKBP possesses a β-sandwich fold that is electrostatically and sterically complementary to its binding partners. Chemokines bind primarily through interactions involving the N-terminal loop and a hydrophobic recess on the ORFV CKBP β-sheet II surface, and largely polar interactions between the chemokine 20s loop and a negatively charged surface groove located at one end of the CKBP β-sheet II surface. ORFV CKBP interacts with leukocyte receptor and glycosaminoglycan binding sites found on the surface of bound chemokines. SEC-MALLS and chromatographic evidence is presented supporting that ORFV CKBP is a dimer in solution over a broad range of protein concentrations. PubMed: 26095031DOI: 10.1016/j.str.2015.04.023 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.15 Å) |
Structure validation
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