4ZKC
The chemokine binding protein of orf virus complexed with CCL7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 93.2 |
Detector technology | CCD |
Collection date | 2011-08-26 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95369 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.430, 77.430, 181.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.220 - 3.150 |
R-factor | 0.26345 |
Rwork | 0.260 |
R-free | 0.32727 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p5i |
RMSD bond length | 0.010 |
RMSD bond angle | 1.467 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.220 | 3.320 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.082 | 0.596 |
Number of reflections | 9921 | |
<I/σ(I)> | 15.7 | 2.3 |
Completeness [%] | 97.4 | 82.1 |
Redundancy | 7.4 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 0.2M potassium sodium tartrate tetrahydrate, 0.1M sodium citrate tribasic dihydrate pH5.6, 2.0M ammonium citrate |