4ZK7

Crystal structure of rescued two-component self-assembling tetrahedral cage T33-31

Summary for 4ZK7

DescriptorChorismate mutase, Divalent-cation tolerance protein CutA (2 entities in total)
Functional Keywordstetrahedral, computational design, rosetta, self-assembly, symmetric, nanomaterial, solubility, protein binding
Biological sourceThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Cellular locationCytoplasm  Q5SJY4 Q7SIA8
Total number of polymer chains24
Total molecular weight324480.56
Authors
Liu, Y.,Cascio, D.,Sawaya, M.R.,Bale, J.,Collazo, M.J.,Park, R.,King, N.,Baker, D.,Yeates, T. (deposition date: 2015-04-30, release date: 2015-07-29, Last modification date: 2017-11-01)
Primary citation
Bale, J.B.,Park, R.U.,Liu, Y.,Gonen, S.,Gonen, T.,Cascio, D.,King, N.P.,Yeates, T.O.,Baker, D.
Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression.
Protein Sci., 24:1695-1701, 2015
PubMed: 26174163 (PDB entries with the same primary citation)
DOI: 10.1002/pro.2748
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.4 Å)
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.24470.1%4.0%1.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution