4Z31

Crystal structure of the RC3H2 ROQ domain in complex with stem-loop and double-stranded forms of RNA

4Z31 の概要

• エントリーDOI: 10.2210/pdb4z31/pdb
• 分子名称: Roquin-2, RNA (5'-R(*A)-D(P*UP*GP*UP*UP*CP*UP*GP*UP*GP*AP*AP*CP*AP*C)-3'), UNKNOWN ATOM OR ION, ... (5 entities in total)
• 機能のキーワード: roquin2, rna, structural genomics, structural genomics consortium, sgc, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm, P-body : Q9HBD1
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 90596.97

構造登録者

DONG, A.,ZHANG, Q.,TEMPEL, W.,Bountra, C.,Arrowsmith, C.H.,Edwards, A.M.,TONG, Y.,Structural Genomics Consortium (SGC) (登録日: 2015-03-30, 公開日: 2015-10-14, 最終更新日: 2023-09-27)

主引用文献

Zhang, Q.,Fan, L.,Hou, F.,Dong, A.,Wang, Y.X.,Tong, Y.
New Insights into the RNA-Binding and E3 Ubiquitin Ligase Activities of Roquins.
Sci Rep, 5:15660-15660, 2015

PubMed Abstract: Roquins are a family of highly conserved RNA-binding proteins that also contain a RING-type E3 ubiquitin ligase domain. They repress constitutive decay elements containing mRNAs and play a critical role in RNA homeostasis and immunological self-tolerance. Here we present the crystal structures of the RNA-binding region of Roquin paralog RC3H2 in both apo- and RNA-bound forms. The RNA-binding region has a bipartite architecture composed of ROQ and HEPN domains, and can bind to stem-loop and double-stranded RNAs simultaneously. The two domains undergo a large orientation change to accommodate RNA duplex binding. We profiled E2 ubiquitin-conjugating enzymes that pair with Roquins and found that RC3H1 and RC3H2 interact with two sets of overlapping but not identical E2 enzymes to drive the assembly of polyubiquitin chains of different linkages. Crystal structures, small-angle X-ray scattering, and E2 profiling revealed that while the two paralogs are highly homologous, RC3H2 and RC3H1 are different in their structures and functions. We also demonstrated that RNA duplex binding to RC3H2 cross-talks with its E3 ubiquitin ligase function using an in vitro auto-ubiquitination assay.

PubMed: 26489670
DOI: 10.1038/srep15660

実験手法

X-RAY DIFFRACTION (2.5 Å)