Loading
PDBj
メニューPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

4YPJ

X-ray Structure of The Mutant of Glycoside Hydrolase

4YPJ の概要
エントリーDOI10.2210/pdb4ypj/pdb
分子名称Beta galactosidase (2 entities in total)
機能のキーワードglycoside hydrolase, hydrolase
由来する生物種Bacillus circulans
タンパク質・核酸の鎖数2
化学式量合計181751.75
構造登録者
Ishikawa, K.,Kataoka, M.,Yanamoto, T.,Nakabayashi, M.,Watanabe, M. (登録日: 2015-03-13, 公開日: 2015-04-29, 最終更新日: 2024-03-20)
主引用文献Ishikawa, K.,Kataoka, M.,Yanamoto, T.,Nakabayashi, M.,Watanabe, M.,Ishihara, S.,Yamaguchi, S.
Crystal structure of beta-galactosidase from Bacillus circulans ATCC 31382 (BgaD) and the construction of the thermophilic mutants.
Febs J., 282:2540-2552, 2015
Cited by
PubMed Abstract: β-Galactosidase (EC 3.2.1.23) from Bacillus circulans ATCC 31382, designated BgaD, exhibits high transglycosylation activity to produce galacto-oligosaccharides. BgaD has been speculated to have a multiple domain architecture including a F5/8-type C domain or a discoidin domain in the C-terminal peptide region from amino acid sequence analysis. Here, we solved the first crystal structure of the C-terminal deletion mutant BgaD-D, consisting of sugar binding, Glyco_hydro, catalytic and bacterial Ig-like domains, at 2.5 Å. In the asymmetric unit, two molecules of BgaD-D were identified and the value of VM was estimated to be 5.0 Å(3) · Da(-1). It has been speculated that BgaD-D consists of four domains. From the structural analysis, however, we clarified that BgaD-D consists of five domains. We identified a new domain structure comprised of β-sheets in BgaD. The catalytic domain exhibits a TIM barrel structure with a small pocket suited for accommodating the disaccharides. Detailed structural information for the amino acid residues related to activity and substrate specificity was clarified in the catalytic domain. Furthermore, using the structural information, we successfully constructed some thermostable mutants via protein engineering method.
PubMed: 25879162
DOI: 10.1111/febs.13298
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.5 Å)
構造検証レポート
Validation report summary of 4ypj
検証レポート(詳細版)ダウンロードをダウンロード

234136

件を2025-04-02に公開中

PDB statisticsPDBj update infoContact PDBjnumon