4YPJ
X-ray Structure of The Mutant of Glycoside Hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-05 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 161.415, 161.415, 275.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 2.500 |
| R-factor | 0.16471 |
| Rwork | 0.163 |
| R-free | 0.19783 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.804 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.162 | 0.479 |
| Number of reflections | 124740 | |
| <I/σ(I)> | 13.5 | 2.9 |
| Completeness [%] | 98.9 | 95.8 |
| Redundancy | 7.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | sodium citrate tribasic dihydrate, sodium acetate trihydrate, PEG 3350 |
