4Y2C

M300V 3D polymerase mutant of EMCV

Summary for 4Y2C

Related4NYZ 4NZ0
DescriptorGenome polyprotein, GLYCEROL (3 entities in total)
Functional Keywordsrna dependent rna polymerase cardiovirus inhibitor resistance, viral protein
Biological sourceMengo encephalomyocarditis virus
Cellular locationProtein 2A: Host nucleus . Protein VP2: Virion. Protein VP3: Virion. Protein VP1: Virion. Protein 2B: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 2C: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3A: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3B: Virion . Protease 3C: Host cytoplasm . RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side  P12296
Total number of polymer chains1
Total molecular weight52580.81
Authors
Verdaguer, N.,Ferrer-Orta, C.,Vives-Adrian, L. (deposition date: 2015-02-09, release date: 2015-04-01)
Primary citation
van der Linden, L.,Vives-Adrian, L.,Selisko, B.,Ferrer-Orta, C.,Liu, X.,Lanke, K.,Ulferts, R.,De Palma, A.M.,Tanchis, F.,Goris, N.,Lefebvre, D.,De Clercq, K.,Leyssen, P.,Lacroix, C.,Purstinger, G.,Coutard, B.,Canard, B.,Boehr, D.D.,Arnold, J.J.,Cameron, C.E.,Verdaguer, N.,Neyts, J.,van Kuppeveld, F.J.
The RNA Template Channel of the RNA-Dependent RNA Polymerase as a Target for Development of Antiviral Therapy of Multiple Genera within a Virus Family.
Plos Pathog., 11:e1004733-e1004733, 2015
PubMed: 25799064 (PDB entries with the same primary citation)
DOI: 10.1371/journal.ppat.1004733
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.2 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.24810 0.5% 3.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
169963
PDB entries from 2020-10-14