4Y2C
M300V 3D polymerase mutant of EMCV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 122.632, 122.632, 198.459 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.360 - 2.200 |
| R-factor | 0.21731 |
| Rwork | 0.216 |
| R-free | 0.24906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nyz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.360 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.081 | 0.665 |
| Number of reflections | 36208 | |
| <I/σ(I)> | 9 | 2 |
| Completeness [%] | 94.5 | 97.4 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10%PEG6000 2.0M NaCl |
