4XW2

Structural basis for simvastatin competitive antagonism of complement receptor 3

4XW2 の概要

• エントリーDOI: 10.2210/pdb4xw2/pdb
• 分子名称: Integrin alpha-M, MAGNESIUM ION, Simvastatin acid, ... (4 entities in total)
• 機能のキーワード: immune system, complement receptor 3, rossmann fold, integrin, statin, protein-inhibitor complex, mac-1, i domain, vwa
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cell membrane ; Single-pass type I membrane protein : P11215
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22941.48

構造登録者

Bajic, G.,Jensen, M.R.,Vorup-Jensen, T.,Andersen, G.R. (登録日: 2015-01-28, 公開日: 2016-01-13, 最終更新日: 2024-01-10)

主引用文献

Jensen, M.R.,Bajic, G.,Zhang, X.,Laustsen, A.K.,Kolds, H.,Skeby, K.K.,Schitt, B.,Andersen, G.R.,Vorup-Jensen, T.
Structural Basis for Simvastatin Competitive Antagonism of Complement Receptor 3.
J.Biol.Chem., 291:16963-16976, 2016

PubMed Abstract: The complement system is an important part of the innate immune response to infection but may also cause severe complications during inflammation. Small molecule antagonists to complement receptor 3 (CR3) have been widely sought, but a structural basis for their mode of action is not available. We report here on the structure of the human CR3 ligand-binding I domain in complex with simvastatin. Simvastatin targets the metal ion-dependent adhesion site of the open, ligand-binding conformation of the CR3 I domain by direct contact with the chelated Mg(2+) ion. Simvastatin antagonizes I domain binding to the complement fragments iC3b and C3d but not to intercellular adhesion molecule-1. By virtue of the I domain's wide distribution in binding kinetics to ligands, it was possible to identify ligand binding kinetics as discriminator for simvastatin antagonism. In static cellular experiments, 15-25 μm simvastatin reduced adhesion by K562 cells expressing recombinant CR3 and by primary human monocytes, with an endogenous expression of this receptor. Application of force to adhering monocytes potentiated the effects of simvastatin where only a 50-100 nm concentration of the drug reduced the adhesion by 20-40% compared with untreated cells. The ability of simvastatin to target CR3 in its ligand binding-activated conformation is a novel mechanism to explain the known anti-inflammatory effects of this compound, in particular because this CR3 conformation is found in pro-inflammatory environments. Our report points to new designs of CR3 antagonists and opens new perspectives and identifies druggable receptors from characterization of the ligand binding kinetics in the presence of antagonists.

PubMed: 27339893
DOI: 10.1074/jbc.M116.732222

実験手法

X-RAY DIFFRACTION (2.001 Å)