4XR3

Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- GC(6) swapped.

4XR3 の概要

• エントリーDOI: 10.2210/pdb4xr3/pdb
• 関連するPDBエントリー: 1ECR 2EWJ 2I05 2I06 4XR0 4XR1 4XR2
• 分子名称: DNA replication terminus site-binding protein, DNA (5'-D(*TP*AP*GP*TP*TP*AP*CP*AP*AP*CP*AP*TP*AP*GP*T)-3'), DNA (5'-D(*TP*AP*CP*TP*AP*TP*GP*TP*TP*GP*TP*AP*AP*CP*TP*A)-3'), ... (6 entities in total)
• 機能のキーワード: dna complex, replication, tus, ter, replication-dna complex, replication/dna
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 47212.46

構造登録者

Oakley, A.J. (登録日: 2015-01-20, 公開日: 2015-08-26, 最終更新日: 2023-09-27)

主引用文献

Elshenawy, M.M.,Jergic, S.,Xu, Z.Q.,Sobhy, M.A.,Takahashi, M.,Oakley, A.J.,Dixon, N.E.,Hamdan, S.M.
Replisome speed determines the efficiency of the Tus-Ter replication termination barrier.
Nature, 525:394-398, 2015

PubMed Abstract: In all domains of life, DNA synthesis occurs bidirectionally from replication origins. Despite variable rates of replication fork progression, fork convergence often occurs at specific sites. Escherichia coli sets a 'replication fork trap' that allows the first arriving fork to enter but not to leave the terminus region. The trap is set by oppositely oriented Tus-bound Ter sites that block forks on approach from only one direction. However, the efficiency of fork blockage by Tus-Ter does not exceed 50% in vivo despite its apparent ability to almost permanently arrest replication forks in vitro. Here we use data from single-molecule DNA replication assays and structural studies to show that both polarity and fork-arrest efficiency are determined by a competition between rates of Tus displacement and rearrangement of Tus-Ter interactions that leads to blockage of slower moving replisomes by two distinct mechanisms. To our knowledge this is the first example where intrinsic differences in rates of individual replisomes have different biological outcomes.

PubMed: 26322585
DOI: 10.1038/nature14866

実験手法

X-RAY DIFFRACTION (2.7 Å)