4XR3

Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- GC(6) swapped.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-17
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95370
Spacegroup name	P 41 21 2
Unit cell lengths	64.069, 64.069, 249.342
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.340 - 2.700
R-factor	0.21341
Rwork	0.210
R-free	0.28466
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i05
RMSD bond length	0.014
RMSD bond angle	1.930
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.106	0.841
Number of reflections	15258
<I/σ(I)>	27.2	3.6
Completeness [%]	99.8	100
Redundancy	13.6	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	8-12% (v/v) PEG 3350, 100 mM NaI, 50 mM Bis-Tris