4XMN

Structure of the yeast coat nucleoporin complex, space group P212121

Summary for 4XMN

• Entry DOI: 10.2210/pdb4xmn/pdb
• Related: 4XMM
• Descriptor: Protein transport protein SEC13, Nucleoporin NUP145, Nucleoporin NUP84, ... (7 entities in total)
• Functional Keywords: structural protein, protein transport
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Cellular location: Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side: Q04491; Nucleoporin NUP145C: Nucleus, nuclear pore complex. Nucleoporin NUP145N: Nucleus, nuclear pore complex: P49687; Nucleus, nuclear pore complex: P52891 P35729 P46673
• Total number of polymer chains: 7
• Total formula weight: 413525.01

Authors

Stuwe, T.,Correia, A.R.,Lin, D.H.,Paduch, M.,Lu, V.T.,Kossiakoff, A.A.,Hoelz, A. (deposition date: 2015-01-14, release date: 2015-03-25, Last modification date: 2024-11-06)

Primary citation

Stuwe, T.,Correia, A.R.,Lin, D.H.,Paduch, M.,Lu, V.T.,Kossiakoff, A.A.,Hoelz, A.
Nuclear pores. Architecture of the nuclear pore complex coat.
Science, 347:1148-1152, 2015

PubMed Abstract: The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. Despite half a century of structural characterization, the architecture of the NPC remains unknown. Here we present the crystal structure of a reconstituted ~400-kilodalton coat nucleoporin complex (CNC) from Saccharomyces cerevisiae at a 7.4 angstrom resolution. The crystal structure revealed a curved Y-shaped architecture and the molecular details of the coat nucleoporin interactions forming the central "triskelion" of the Y. A structural comparison of the yeast CNC with an electron microscopy reconstruction of its human counterpart suggested the evolutionary conservation of the elucidated architecture. Moreover, 32 copies of the CNC crystal structure docked readily into a cryoelectron tomographic reconstruction of the fully assembled human NPC, thereby accounting for ~16 megadalton of its mass.

PubMed: 25745173
DOI: 10.1126/science.aaa4136

Experimental method

X-RAY DIFFRACTION (7.6 Å)