4XMN

Structure of the yeast coat nucleoporin complex, space group P212121

4XMN の概要

• エントリーDOI: 10.2210/pdb4xmn/pdb
• 関連するPDBエントリー: 4XMM
• 分子名称: Protein transport protein SEC13, Nucleoporin NUP145, Nucleoporin NUP84, ... (7 entities in total)
• 機能のキーワード: structural protein, protein transport
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• 細胞内の位置: Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side: Q04491; Nucleoporin NUP145C: Nucleus, nuclear pore complex. Nucleoporin NUP145N: Nucleus, nuclear pore complex: P49687; Nucleus, nuclear pore complex: P52891 P35729 P46673
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 413525.01

構造登録者

Stuwe, T.,Correia, A.R.,Lin, D.H.,Paduch, M.,Lu, V.T.,Kossiakoff, A.A.,Hoelz, A. (登録日: 2015-01-14, 公開日: 2015-03-25, 最終更新日: 2024-11-06)

主引用文献

Stuwe, T.,Correia, A.R.,Lin, D.H.,Paduch, M.,Lu, V.T.,Kossiakoff, A.A.,Hoelz, A.
Nuclear pores. Architecture of the nuclear pore complex coat.
Science, 347:1148-1152, 2015

PubMed Abstract: The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. Despite half a century of structural characterization, the architecture of the NPC remains unknown. Here we present the crystal structure of a reconstituted ~400-kilodalton coat nucleoporin complex (CNC) from Saccharomyces cerevisiae at a 7.4 angstrom resolution. The crystal structure revealed a curved Y-shaped architecture and the molecular details of the coat nucleoporin interactions forming the central "triskelion" of the Y. A structural comparison of the yeast CNC with an electron microscopy reconstruction of its human counterpart suggested the evolutionary conservation of the elucidated architecture. Moreover, 32 copies of the CNC crystal structure docked readily into a cryoelectron tomographic reconstruction of the fully assembled human NPC, thereby accounting for ~16 megadalton of its mass.

PubMed: 25745173
DOI: 10.1126/science.aaa4136

実験手法

X-RAY DIFFRACTION (7.6 Å)