4XLP

Crystal structure of T.aquaticus transcription initiation complex containing upstream fork promoter

4XLP の概要

• エントリーDOI: 10.2210/pdb4xlp/pdb
• 関連するPDBエントリー: 4XAX 4XLN 4XLQ 4XLR 4XLS
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total)
• 機能のキーワード: protein-dna complex, bacterial transcription initiation complex, transcription, transcription-dna complex, transcription/dna
• 由来する生物種: Thermus aquaticus; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 868637.10

構造登録者

Bae, B.,Darst, S.A. (登録日: 2015-01-13, 公開日: 2015-09-23, 最終更新日: 2024-11-13)

主引用文献

Bae, B.,Feklistov, A.,Lass-Napiorkowska, A.,Landick, R.,Darst, S.A.
Structure of a bacterial RNA polymerase holoenzyme open promoter complex.
Elife, 4:-, 2015

PubMed Abstract: Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo). We have determined crystal structures, refined to 4.14 Å-resolution, of RPo containing Thermus aquaticus RNAP holoenzyme and promoter DNA that includes the full transcription bubble. The structures, combined with biochemical analyses, reveal key features supporting the formation and maintenance of the double-strand/single-strand DNA junction at the upstream edge of the -10 element where bubble formation initiates. The results also reveal RNAP interactions with duplex DNA just upstream of the -10 element and potential protein/DNA interactions that direct the DNA template strand into the RNAP active site. Addition of an RNA primer to yield a 4 base-pair post-translocated RNA:DNA hybrid mimics an initially transcribing complex at the point where steric clash initiates abortive initiation and σ(A) dissociation.

PubMed: 26349032
DOI: 10.7554/eLife.08504

実験手法

X-RAY DIFFRACTION (4 Å)