4XLP
Crystal structure of T.aquaticus transcription initiation complex containing upstream fork promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 289.870, 289.870, 537.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.030 - 4.000 |
| R-factor | 0.2588 |
| Rwork | 0.257 |
| R-free | 0.29730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.911 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.030 | 45.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 8.600 | 4.000 |
| Rmerge | 0.191 | 0.097 | |
| Total number of observations | 2102998 | ||
| Number of reflections | 191386 | ||
| <I/σ(I)> | 2.3 | ||
| Completeness [%] | 100.0 | 99.9 | 99.7 |
| Redundancy | 11 | 11.1 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | ammonium sulfate, magnesium chloride, Tris-HCl |
