4X4I

RADIATION DAMAGE TO THE NUCLEOPROTEIN COMPLEX C.Esp1396I: DOSE (DWD) 44.6 MGy

Summary for 4X4I

• Entry DOI: 10.2210/pdb4x4i/pdb
• Related: 3CLC 4X4B 4X4C 4X4D 4X4E 4X4F 4X4G 4X4H
• Descriptor: Regulatory protein, 35-MER DNA (3 entities in total)
• Functional Keywords: protein-dna complex, radiation damage, gene regulation
• Biological source: Enterobacter sp. RFL1396; More
• Total number of polymer chains: 6
• Total formula weight: 59615.63

Authors

Bury, C.S.,McGeehan, J.E.,Garman, E.F. (deposition date: 2014-12-02, release date: 2015-03-11, Last modification date: 2024-01-10)

Primary citation

Bury, C.,Garman, E.F.,Ginn, H.M.,Ravelli, R.B.,Carmichael, I.,Kneale, G.,McGeehan, J.E.
Radiation damage to nucleoprotein complexes in macromolecular crystallography.
J.Synchrotron Radiat., 22:213-224, 2015

PubMed Abstract: Significant progress has been made in macromolecular crystallography over recent years in both the understanding and mitigation of X-ray induced radiation damage when collecting diffraction data from crystalline proteins. In contrast, despite the large field that is productively engaged in the study of radiation chemistry of nucleic acids, particularly of DNA, there are currently very few X-ray crystallographic studies on radiation damage mechanisms in nucleic acids. Quantitative comparison of damage to protein and DNA crystals separately is challenging, but many of the issues are circumvented by studying pre-formed biological nucleoprotein complexes where direct comparison of each component can be made under the same controlled conditions. Here a model protein-DNA complex C.Esp1396I is employed to investigate specific damage mechanisms for protein and DNA in a biologically relevant complex over a large dose range (2.07-44.63 MGy). In order to allow a quantitative analysis of radiation damage sites from a complex series of macromolecular diffraction data, a computational method has been developed that is generally applicable to the field. Typical specific damage was observed for both the protein on particular amino acids and for the DNA on, for example, the cleavage of base-sugar N1-C and sugar-phosphate C-O bonds. Strikingly the DNA component was determined to be far more resistant to specific damage than the protein for the investigated dose range. At low doses the protein was observed to be susceptible to radiation damage while the DNA was far more resistant, damage only being observed at significantly higher doses.

PubMed: 25723923
DOI: 10.1107/S1600577514026289

Experimental method

X-RAY DIFFRACTION (2.8 Å)