4WUR

The crystal structure of the MERS-CoV papain-like protease (C111S) with human ubiquitin

4WUR の概要

• エントリーDOI: 10.2210/pdb4wur/pdb
• 関連するPDBエントリー: 4P16
• 分子名称: Papain-like protease, Polyubiquitin-B, ISOPROPYL ALCOHOL, ... (4 entities in total)
• 機能のキーワード: mers-cov, papain-like protease, ubiquitin, complex, hydrolase
• 由来する生物種: Human coronavirus EMC (HCoV-EMC); 詳細
• 細胞内の位置: Papain-like proteinase: Host membrane; Multi-pass membrane protein. Non-structural protein 4: Host membrane; Multi-pass membrane protein. Non-structural protein 6: Host membrane ; Multi-pass membrane protein . Non-structural protein 7: Host cytoplasm, host perinuclear region . Non-structural protein 8: Host cytoplasm, host perinuclear region . Non-structural protein 9: Host cytoplasm, host perinuclear region . Non-structural protein 10: Host cytoplasm, host perinuclear region . Helicase: Host endoplasmic reticulum-Golgi intermediate compartment . Uridylate-specific endoribonuclease: Host cytoplasm, host perinuclear region : K9N7C7; Ubiquitin: Cytoplasm : P0CG47
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44917.97

構造登録者

Lei, J.,Hilgenfeld, R. (登録日: 2014-11-03, 公開日: 2014-11-26, 最終更新日: 2024-01-10)

主引用文献

Lei, J.,Hilgenfeld, R.
Structural and mutational analysis of the interaction between the Middle-East respiratory syndrome coronavirus (MERS-CoV) papain-like protease and human ubiquitin.
Virol Sin, 31:288-299, 2016

PubMed Abstract: The papain-like protease (PL(pro)) of Middle-East respiratory syndrome coronavirus (MERS-CoV) has proteolytic, deubiquitinating, and deISGylating activities. The latter two are involved in the suppression of the antiviral innate immune response of the host cell. To contribute to an understanding of this process, we present here the X-ray crystal structure of a complex between MERS-CoV PL(pro) and human ubiquitin (Ub) that is devoid of any covalent linkage between the two proteins. Five regions of the PL(pro) bind to two areas of the Ub. The C-terminal five residues of Ub, RLRGG, are similar to the P5-P1 residues of the polyprotein substrates of the PL(pro) and are responsible for the major part of the interaction between the two macromolecules. Through sitedirected mutagenesis, we demonstrate that conserved Asp165 and non-conserved Asp164 are important for the catalytic activities of MERS-CoV PL(pro). The enzyme appears not to be optimized for catalytic efficiency; thus, replacement of Phe269 by Tyr leads to increased peptidolytic and deubiquitinating activities. Ubiquitin binding by MERS-CoV PL(pro) involves remarkable differences compared to the corresponding complex with SARS-CoV PL(pro). The structure and the mutational study help understand common and unique features of the deubiquitinating activity of MERS-CoV PL(pro).

PubMed: 27245450
DOI: 10.1007/s12250-016-3742-4

実験手法

X-RAY DIFFRACTION (3.16 Å)