4WTI

CRYSTAL STRUCTURE OF HCV NS5B GENOTYPE 2A JFH-1 ISOLATE WITH S15G E86Q E87Q C223H V321I MUTATIONS IN COMPLEX WITH RNA TEMPLATE 5'-ACGG, RNA PRIMER 5'-PCC, MN2+, AND GDP

Summary for 4WTI

• Entry DOI: 10.2210/pdb4wti/pdb
• Descriptor: RNA TEMPLATE ACGG, RNA PRIMER CC, RNA-directed RNA polymerase, ... (8 entities in total)
• Functional Keywords: hcv, viral, ns5b, rdrp, resistance mutation, template, primer, primed initiation, transferase-rna complex, transferase/rna
• Biological source: Hepatitis C virus JFH-1; More
• Cellular location: Core protein p21: Host endoplasmic reticulum membrane ; Single-pass membrane protein . Core protein p19: Virion . Envelope glycoprotein E1: Virion membrane ; Single-pass type I membrane protein . Envelope glycoprotein E2: Virion membrane ; Single-pass type I membrane protein . p7: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Protease NS2-3: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein . Non-structural protein 4A: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein . Non-structural protein 4B: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Non-structural protein 5A: Host endoplasmic reticulum membrane ; Peripheral membrane protein . RNA-directed RNA polymerase: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein : Q99IB8
• Total number of polymer chains: 3
• Total formula weight: 67590.43

Authors

Edwards, T.E.,Appleby, T.C. (deposition date: 2014-10-30, release date: 2015-02-11, Last modification date: 2023-09-27)

• Primary citation: Appleby, T.C.,Perry, J.K.,Murakami, E.,Barauskas, O.,Feng, J.,Cho, A.,Fox, D.,Wetmore, D.R.,McGrath, M.E.,Ray, A.S.,Sofia, M.J.,Swaminathan, S.,Edwards, T.E.; Structural basis for RNA replication by the hepatitis C virus polymerase.; Science, 347:771-775, 2015; PubMed: 25678663; DOI: 10.1126/science.1259210

Experimental method

X-RAY DIFFRACTION (2.8 Å)