4WTI
CRYSTAL STRUCTURE OF HCV NS5B GENOTYPE 2A JFH-1 ISOLATE WITH S15G E86Q E87Q C223H V321I MUTATIONS IN COMPLEX WITH RNA TEMPLATE 5'-ACGG, RNA PRIMER 5'-PCC, MN2+, AND GDP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (T) | RNA TEMPLATE ACGG | polymer | 4 | 1279.8 | 1 | synthetic construct | ||
| 2 | B (P) | RNA PRIMER CC | polymer | 2 | 565.4 | 1 | synthetic construct | ||
| 3 | C (A) | RNA-directed RNA polymerase | polymer | 580 | 64819.4 | 1 | UniProt (Q99IB8) | Hepatitis C virus JFH-1 | |
| 4 | D, E, F (A) | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) | |||
| 5 | G (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | H (A) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 7 | I (A) | 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 282.3 | 1 | Chemie (B3P) | |||
| 8 | J, K (P, A) | water | water | 18.0 | 62 | Chemie (HOH) |
Sequence modifications
A: 1 - 570 (UniProt: Q99IB8)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 15 | Ser 2457 | engineered mutation |
| Gln 86 | Glu 2528 | engineered mutation |
| Gln 87 | Glu 2529 | engineered mutation |
| His 223 | Cys 2665 | engineered mutation |
| Ile 321 | Val 2763 | engineered mutation |
| Leu 571 | - | expression tag |
| Glu 572 | - | expression tag |
| His 573 | - | expression tag |
| His 574 | - | expression tag |
| His 575 | - | expression tag |
| His 576 | - | expression tag |
| His 577 | - | expression tag |
| His 578 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 66664.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 925.8 | |
| All* | Total formula weight | 67590.4 |
