4WP5

Chaetomium thermophilum Mex67 NTF2-like domain complexed with Mtr2

Summary for 4WP5

• Entry DOI: 10.2210/pdb4wp5/pdb
• Descriptor: mRNA export protein, Mtr2 (3 entities in total)
• Functional Keywords: nuclear export factor, transport protein
• Biological source: Chaetomium thermophilum; More
• Total number of polymer chains: 2
• Total formula weight: 44735.33

Authors

Aibara, S.,Valkov, E.,Stewart, M. (deposition date: 2014-10-17, release date: 2015-07-15, Last modification date: 2024-05-08)

Primary citation

Aibara, S.,Valkov, E.,Lamers, M.H.,Dimitrova, L.,Hurt, E.,Stewart, M.
Structural characterization of the principal mRNA-export factor Mex67-Mtr2 from Chaetomium thermophilum.
Acta Crystallogr.,Sect.F, 71:876-888, 2015

PubMed Abstract: Members of the Mex67-Mtr2/NXF-NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, especially in lower eukaryotes, is low. Here, the crystal structures of these domains from the thermophilic fungus Chaetomium thermophilum are presented together with small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate that, not withstanding the limited sequence conservation between different NXF family members, the molecules retain similar structural and RNA-binding properties. Moreover, the resolution of crystal structures obtained with the C. thermophilum domains was often higher than that obtained previously and, when combined with solution and biochemical studies, provided insight into the structural organization, self-association and RNA-binding properties of Mex67-Mtr2 that facilitate mRNA nuclear export.

PubMed: 26144233
DOI: 10.1107/S2053230X15008766

Experimental method

X-RAY DIFFRACTION (2.9 Å)