4WOB
Proteinase-K Pre-Surface Acoustic Wave
Summary for 4WOB
| Entry DOI | 10.2210/pdb4wob/pdb |
| Related | 4WO6 4WO9 4WOA 4WOC |
| Descriptor | Proteinase K, SULFATE ION (3 entities in total) |
| Functional Keywords | surface acoustic wave, nanocrystals, crystal manipulation, serial crystallography, acoustic tweezers, hydrolase |
| Biological source | Parengyodontium album (Tritirachium album) |
| Total number of polymer chains | 1 |
| Total formula weight | 29054.85 |
| Authors | French, J.B. (deposition date: 2014-10-15, release date: 2015-02-18, Last modification date: 2024-11-20) |
| Primary citation | Guo, F.,Zhou, W.,Li, P.,Mao, Z.,Yennawar, N.H.,French, J.B.,Huang, T.J. Precise Manipulation and Patterning of Protein Crystals for Macromolecular Crystallography Using Surface Acoustic Waves. Small, 11:2733-2737, 2015 Cited by PubMed Abstract: Advances in modern X-ray sources and detector technology have made it possible for crystallographers to collect usable data on crystals of only a few micrometers or less in size. Despite these developments, sample handling techniques have significantly lagged behind and often prevent the full realization of current beamline capabilities. In order to address this shortcoming, a surface acoustic wave-based method for manipulating and patterning crystals is developed. This method, which does not damage the fragile protein crystals, can precisely manipulate and pattern micrometer and submicrometer-sized crystals for data collection and screening. The technique is robust, inexpensive, and easy to implement. This method not only promises to significantly increase efficiency and throughput of both conventional and serial crystallography experiments, but will also make it possible to collect data on samples that were previously intractable. PubMed: 25641793DOI: 10.1002/smll.201403262 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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