4WC4

tRNA-processing enzyme complex 2

Summary for 4WC4

• Entry DOI: 10.2210/pdb4wc4/pdb
• Related: 1VFG 4WBY 4WBZ 4WC0 4WC1 4WC2 4WC3 4WC5 4WC6 4WC7
• Descriptor: Poly A polymerase, RNA (74-MER), ADENOSINE-5'-TRIPHOSPHATE (3 entities in total)
• Functional Keywords: rna nucleotidyltransferase, cca-adding enzyme, a-adding enzyme, transferase-rna complex, transferase/rna
• Biological source: Aquifex aeolicus; More
• Total number of polymer chains: 2
• Total formula weight: 70908.06

Authors

Yamashita, S.,Tomita, K. (deposition date: 2014-09-04, release date: 2015-04-15, Last modification date: 2024-03-20)

Primary citation

Yamashita, S.,Martinez, A.,Tomita, K.
Measurement of Acceptor-T Psi C Helix Length of tRNA for Terminal A76-Addition by A-Adding Enzyme.
Structure, 23:830-842, 2015

PubMed Abstract: The 3'-terminal CCA (C74C75A76-3') of tRNA is required for protein synthesis. In Aquifex aeolicus, the CCA-3' is synthesized by CC-adding and A-adding enzymes, although in most organisms, CCA is synthesized by a single CCA-adding enzyme. The mechanisms by which the A-adding enzyme adds only A76, but not C74C75, onto tRNA remained elusive. The complex structures of the enzyme with various tRNAs revealed the presence of a single tRNA binding site on the enzyme, with the enzyme measuring the acceptor-TΨC helix length of tRNA. The 3'-C75 of tRNA lacking A76 can reach the active site and the size and shape of the nucleotide binding pocket at the insertion stage are suitable for ATP. The 3'-C74 of tRNA lacking C75A76 cannot reach the active site, although CTP or ATP can bind the active pocket. Thus, the A-adding enzyme adds only A76, but not C74C75, onto tRNA.

PubMed: 25914059
DOI: 10.1016/j.str.2015.03.013

Experimental method

X-RAY DIFFRACTION (3.501 Å)