4WC4
tRNA-processing enzyme complex 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2014-06-18 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.310, 111.760, 127.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.881 - 3.501 |
R-factor | 0.2782 |
Rwork | 0.277 |
R-free | 0.30200 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.492 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.590 | |
High resolution limit [Å] | 3.500 | 15.660 | 3.500 |
Rmerge | 0.222 | 0.039 | 1.309 |
Rmeas | 0.239 | 0.043 | 1.410 |
Total number of observations | 80315 | ||
Number of reflections | 11158 | 74 | 797 |
<I/σ(I)> | 8.94 | 31.27 | 1.86 |
Completeness [%] | 99.2 | 47.4 | 99.4 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | isopropanol, potassium chloride, magnesium chloride, sodium cacodylate |