4WA8

Methanopyrus Kandleri FEN-1 nuclease

Summary for 4WA8

• Entry DOI: 10.2210/pdb4wa8/pdb
• Descriptor: Flap endonuclease 1, CHLORIDE ION (3 entities in total)
• Functional Keywords: dna nuclease, nucleotide excision repair, hydrolase
• Biological source: Methanopyrus kandleri
• Total number of polymer chains: 2
• Total formula weight: 82178.87

Authors

Shah, S.,Dunten, P.,Horton, N.C. (deposition date: 2014-08-28, release date: 2014-11-12, Last modification date: 2023-09-27)

Primary citation

Shah, S.,Dunten, P.,Stiteler, A.,Park, C.K.,Horton, N.C.
Structure and specificity of FEN-1 from Methanopyrus kandleri.
Proteins, 83:188-194, 2015

PubMed Abstract: DNA repair is fundamental to genome stability and is found in all three domains of life. However many archaeal species, such as Methanopyrus kandleri, contain only a subset of the eukaryotic nucleotide excision repair (NER) homologs, and those present often contain significant differences compared to their eukaryotic homologs. To clarify the role of the NER XPG-like protein Mk0566 from M. kandleri, its biochemical activity and three-dimensional structure were investigated. Both were found to be more similar to human FEN-1 than human XPG, suggesting a biological role in replication and long-patch base excision repair rather than in NER.

PubMed: 25354467
DOI: 10.1002/prot.24704

Experimental method

X-RAY DIFFRACTION (2.2 Å)