4W99

Apo-structure of the Y79F,W322E-double mutant of Etr1p

Summary for 4W99

• Entry DOI: 10.2210/pdb4w99/pdb
• Related: 1N9G
• Descriptor: Enoyl-[acyl-carrier-protein] reductase [NADPH, B-specific] 1, mitochondrial, GLYCEROL, SULFATE ION, ... (4 entities in total)
• Functional Keywords: reductase, mitochondrial fatty acid synthesis, oxidoreductase
• Biological source: Candida tropicalis (Yeast)
• Cellular location: Mitochondrion : Q8WZM3
• Total number of polymer chains: 2
• Total formula weight: 79913.33

Authors

Quade, N.,Voegeli, B.,Rosenthal, R.,Erb, T.J. (deposition date: 2014-08-27, release date: 2015-03-18, Last modification date: 2024-05-08)

Primary citation

Rosenthal, R.G.,Vogeli, B.,Quade, N.,Capitani, G.,Kiefer, P.,Vorholt, J.A.,Ebert, M.O.,Erb, T.J.
The use of ene adducts to study and engineer enoyl-thioester reductases.
Nat.Chem.Biol., 11:398-400, 2015

PubMed Abstract: An improved understanding of enzymes' catalytic proficiency and stereoselectivity would further enable applications in chemistry, biocatalysis and industrial biotechnology. We use a chemical probe to dissect individual catalytic steps of enoyl-thioester reductases (Etrs), validating an active site tyrosine as the cryptic proton donor and explaining how it had eluded definitive identification. This information enabled the rational redesign of Etr, yielding mutants that create products with inverted stereochemistry at wild type-like turnover frequency.

PubMed: 25867044
DOI: 10.1038/nchembio.1794

Experimental method

X-RAY DIFFRACTION (2 Å)