4W99
Apo-structure of the Y79F,W322E-double mutant of Etr1p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2013-11-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.335, 100.933, 80.537 |
Unit cell angles | 90.00, 102.56, 90.00 |
Refinement procedure
Resolution | 39.305 - 2.000 |
R-factor | 0.1735 |
Rwork | 0.171 |
R-free | 0.21410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 | |
High resolution limit [Å] | 2.000 | 8.920 | 2.000 |
Rmerge | 0.046 | 0.023 | 0.631 |
Rmeas | 0.056 | 0.028 | 0.779 |
Total number of observations | 209984 | ||
Number of reflections | 69226 | 811 | 4869 |
<I/σ(I)> | 12.76 | 33.16 | 1.59 |
Completeness [%] | 98.2 | 98.1 | 93.5 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 2M ammonium sulfate, 0.1M ADA/NaOH pH 7 |