4W8J

Structure of the full-length insecticidal protein Cry1Ac reveals intriguing details of toxin packaging into in vivo formed crystals

Summary for 4W8J

Entry DOI	10.2210/pdb4w8j/pdb
Descriptor	Pesticidal crystal protein cry1Ac, POTASSIUM ION, CALCIUM ION, ... (4 entities in total)
Functional Keywords	toxin, insecticidal, pore forming, protoxin
Biological source	Bacillus thuringiensis serovar kurstaki str. HD73
Total number of polymer chains	1
Total formula weight	134138.40
Authors	Evdokimov, A.G.,Moshiri, F.,Sturman, E.J.,Rydel, T.J.,Zheng, M.,Seale, J.W.,Franklin, S. (deposition date: 2014-08-25, release date: 2014-09-17, Last modification date: 2023-12-27)
Primary citation	Evdokimov, A.G.,Moshiri, F.,Sturman, E.J.,Rydel, T.J.,Zheng, M.,Seale, J.W.,Franklin, S. Structure of the full-length insecticidal protein Cry1Ac reveals intriguing details of toxin packaging into in vivo formed crystals. Protein Sci., 23:1491-1497, 2014 Cited by PubMed Abstract: For almost half a century, the structure of the full-length Bacillus thuringiensis (Bt) insecticidal protein Cry1Ac has eluded researchers, since Bt-derived crystals were first characterized in 1965. Having finally solved this structure we report intriguing details of the lattice-based interactions between the toxic core of the protein and the protoxin domains. The structure provides concrete evidence for the function of the protoxin as an enhancer of native crystal packing and stability. PubMed: 25139047 DOI: 10.1002/pro.2536 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.78 Å)

Structure validation