4W8J

Structure of the full-length insecticidal protein Cry1Ac reveals intriguing details of toxin packaging into in vivo formed crystals

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Pesticidal crystal protein cry1Ac	polymer	1184	133981.0	1	UniProt (M4LET9) Pfam (PF03945) Pfam (PF00555) Pfam (PF03944) Pfam (PF17997) Pfam (PF21463) UniProt (by SIFTS) (P05068) In PDB	Bacillus thuringiensis serovar kurstaki str. HD73
2	A	POTASSIUM ION	non-polymer		39.1	3	Chemie (K)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4		water	water		18.0	50	Chemie (HOH)

Sequence modifications

A: 1 - 1178 (UniProt: M4LET9)

PDB	External Database	Details
Leu 148	Phe 148	conflict
Val 462	Phe 462	conflict
Ser 661	Cys 661	engineered mutation
Ser 730	Cys 730	engineered mutation
Ser 796	Cys 796	engineered mutation
Ser 802	Cys 802	engineered mutation
Ser 814	Cys 814	engineered mutation
Ser 816	Cys 816	engineered mutation
Ser 822	Cys 822	engineered mutation
Ser 837	Cys 837	engineered mutation
Ser 990	Cys 990	engineered mutation
Ser 1025	Cys 1025	engineered mutation
Ser 1045	Cys 1045	engineered mutation
Ser 1063	Cys 1063	engineered mutation
Ser 1076	Cys 1076	engineered mutation
Ser 1125	Cys 1125	engineered mutation
His 1179	-	expression tag
His 1180	-	expression tag
His 1181	-	expression tag
His 1182	-	expression tag
His 1183	-	expression tag
His 1184	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	133981.0
Non-Polymers*	Number of molecules	4
	Total formula weight	157.4
All*	Total formula weight	134138.4

*Water molecules are not included.