Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (soaked), mRNA and three deacylated tRNAs in the A, P and E sites

This is a large structure.

Summary for 4W2G

Integrates4RB9 4RBA 4RBB 4RBC
Descriptor16S Ribosomal RNA, 30S Ribosomal Protein S2, 30S Ribosomal Protein S3, ... (61 entities in total)
Functional Keywordspactamycin, antibiotic, 70s ribosome, inhibition of translation, initiation inhibitor, 30s subunit with pactamycin (soaked), mrna, deacylated a-, p-, and e-site trnas of the 1st 70s ribosome in the asu, functional complex of bacterial 70s ribosome with antibiotic pactamycin (compact conformation), 50s subunit of the 1st 70s ribosome in the asu, ribosome-antibiotic complex, ribosome/antibiotic
Biological sourceEscherichia coli
Total number of polymer chains112
Total molecular weight4554636.62
Primary citation
Polikanov, Y.S.,Osterman, I.A.,Szal, T.,Tashlitsky, V.N.,Serebryakova, M.V.,Kusochek, P.,Bulkley, D.,Malanicheva, I.A.,Efimenko, T.A.,Efremenkova, O.V.,Konevega, A.L.,Shaw, K.J.,Bogdanov, A.A.,Rodnina, M.V.,Dontsova, O.A.,Mankin, A.S.,Steitz, T.A.,Sergiev, P.V.
Amicoumacin a inhibits translation by stabilizing mRNA interaction with the ribosome.
Mol.Cell, 56:531-540, 2014
PubMed: 25306919 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2014.09.020
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.274101.2%8.5%8.4%0.51MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 4w2g
no rotation
Molmil generated image of 4w2g
rotated about x axis by 90°
Molmil generated image of 4w2g
rotated about y axis by 90°
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