4W2G
Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (soaked), mRNA and three deacylated tRNAs in the A, P and E sites
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.350, 449.010, 621.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 145.516 - 2.550 |
R-factor | 0.2276 |
Rwork | 0.225 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.033 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 310.990 | 2.620 | |
High resolution limit [Å] | 2.550 | 11.400 | 2.550 |
Rmerge | 0.139 | 0.012 | 0.012 |
Rmeas | 0.033 | 1.480 | |
Number of reflections | 1857656 | 21042 | 137425 |
<I/σ(I)> | 7.61 | 26.63 | 1 |
Completeness [%] | 99.0 | 95 | 99.4 |
Redundancy | 3.723 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K |