4V9I

Crystal structure of thermus thermophilus 70S in complex with tRNAs and mRNA containing a pseudouridine in a stop codon

This is a non-PDB format compatible entry.

Summary for 4V9I

• Entry DOI: 10.2210/pdb4v9i/pdb
• Descriptor: 16S ribosomal RNA, 30S Ribosomal protein S10, 30S Ribosomal protein S11, ... (60 entities in total)
• Functional Keywords: ribosomal subunit, translation, ribosome
• Biological source: Thermus thermophilus; More
• Total number of polymer chains: 116
• Total formula weight: 4454288.17

Authors

Fernandez, I.S.,Ng, C.L.,Kelley, A.C.,Guowei, W.,Yu, Y.T.,Ramakrishnan, V. (deposition date: 2013-04-04, release date: 2014-07-09, Last modification date: 2014-12-10)

Primary citation

Fernandez, I.S.,Ng, C.L.,Kelley, A.C.,Wu, G.,Yu, Y.T.,Ramakrishnan, V.
Unusual base pairing during the decoding of a stop codon by the ribosome.
Nature, 500:107-110, 2013

PubMed Abstract: During normal translation, the binding of a release factor to one of the three stop codons (UGA, UAA or UAG) results in the termination of protein synthesis. However, modification of the initial uridine to a pseudouridine (Ψ) allows efficient recognition and read-through of these stop codons by a transfer RNA (tRNA), although it requires the formation of two normally forbidden purine-purine base pairs. Here we determined the crystal structure at 3.1 Å resolution of the 30S ribosomal subunit in complex with the anticodon stem loop of tRNA(Ser) bound to the ΨAG stop codon in the A site. The ΨA base pair at the first position is accompanied by the formation of purine-purine base pairs at the second and third positions of the codon, which show an unusual Watson-Crick/Hoogsteen geometry. The structure shows a previously unsuspected ability of the ribosomal decoding centre to accommodate non-canonical base pairs.

PubMed: 23812587
DOI: 10.1038/nature12302

Experimental method

X-RAY DIFFRACTION (3.3 Å)