4V9I

Crystal structure of thermus thermophilus 70S in complex with tRNAs and mRNA containing a pseudouridine in a stop codon

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, GB (AA, CA)	16S ribosomal RNA	polymer	1504	488391.2	2	GenBank (55771382)	Thermus thermophilus
2	B, HB (AB, CB)	30S Ribosomal protein S2	polymer	234	26987.3	2	UniProt (P80371) Pfam (PF00318)	Thermus thermophilus
3	C, IB (AC, CC)	30S Ribosomal protein S3	polymer	206	22862.4	2	UniProt (P80372) Pfam (PF07650) Pfam (PF00189)	Thermus thermophilus
4	D, JB (AD, CD)	30S Ribosomal protein S4	polymer	208	24242.3	2	UniProt (P80373) Pfam (PF00163) Pfam (PF01479)	Thermus thermophilus
5	E, KB (AE, CE)	30S Ribosomal protein S5	polymer	150	16331.1	2	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719)	Thermus thermophilus
6	F, LB (AF, CF)	30S Ribosomal protein S6	polymer	101	11988.8	2	UniProt (Q5SLP8) Pfam (PF01250)	Thermus thermophilus
7	G, MB (AG, CG)	30S Ribosomal protein S7	polymer	155	17919.8	2	UniProt (P17291) Pfam (PF00177)	Thermus thermophilus
8	H, NB (AH, CH)	30S Ribosomal protein S8	polymer	138	15868.6	2	UniProt (Q5SHQ2)	Thermus thermophilus
9	I, OB (AI, CI)	30S Ribosomal protein S9	polymer	127	14298.5	2	UniProt (P80374) Pfam (PF00380)	Thermus thermophilus
10	J, PB (AJ, CJ)	30S Ribosomal protein S10	polymer	98	11299.2	2	UniProt (Q5SHN7) Pfam (PF00338)	Thermus thermophilus
11	K, QB (AK, CK)	30S Ribosomal protein S11	polymer	119	12606.4	2	UniProt (P80376) Pfam (PF00411)	Thermus thermophilus
12	L, RB (AL, CL)	30S Ribosomal protein S12	polymer	124	13804.3	2	UniProt (Q5SHN3) Pfam (PF00164)	Thermus thermophilus
13	M, SB (AM, CM)	30S Ribosomal protein S13	polymer	124	14078.5	2	UniProt (P80377) Pfam (PF00416)	Thermus thermophilus
14	N, TB (AN, CN)	30S Ribosomal protein S14	polymer	60	7027.5	2	UniProt (Q5SHQ1)	Thermus thermophilus
15	O, UB (AO, CO)	30S Ribosomal protein S15	polymer	88	10447.2	2	UniProt (Q5SJ76) Pfam (PF00312)	Thermus thermophilus
16	P, VB (AP, CP)	30S Ribosomal protein S16	polymer	83	9924.5	2	UniProt (Q5SJH3) Pfam (PF00886)	Thermus thermophilus
17	Q, WB (AQ, CQ)	30S Ribosomal protein S17	polymer	99	11722.9	2	UniProt (Q5SHP7)	Thermus thermophilus
18	R, XB (AR, CR)	30S Ribosomal protein S18	polymer	70	8155.8	2	UniProt (Q5SLQ0) Pfam (PF01084)	Thermus thermophilus
19	S, YB (AS, CS)	30S Ribosomal protein S19	polymer	78	8949.4	2	UniProt (Q5SHP2) Pfam (PF00203)	Thermus thermophilus
20	T, ZB (AT, CT)	30S Ribosomal protein S20	polymer	99	10921.1	2	UniProt (P80380) Pfam (PF01649)	Thermus thermophilus
21	AC, U (AU, CU)	30S Ribosomal protein THX	polymer	24	2960.5	2	UniProt (Q5SIH3) Pfam (PF17070)	Thermus thermophilus
22	BC, V (AV, CV)	P-SITE tRNA	polymer	77	24802.8	2	GenBank (443419838)	Escherichia coli
23	CC, W (AW, CW)	E-SITE tRNA	polymer	76	24485.5	2	GenBank (443419838)	Escherichia coli
24	DC, X (AY, CY)	A-SITE tRNA	polymer	75	24430.6	2	GenBank (443419838)	Escherichia coli
25	Y (AX)	mRNA	polymer	7	2245.4	1		Synthetic construct
26	FC, Z (BA, DA)	23S ribosomal RNA	polymer	2915	947919.4	2	GenBank (55771382)	Thermus thermophilus
27	AA, GC (BB, DB)	5S ribosomal RNA	polymer	119	38553.0	2	GenBank (55771382)	Thermus thermophilus
28	BA (BC)	50S ribosomal protein L1	polymer	206	17813.9	1		Thermus thermophilus
29	CA, IC (BD, DD)	50S ribosomal protein L2	polymer	271	29828.6	2	UniProt (P60405) Pfam (PF00181) Pfam (PF03947)	Thermus thermophilus
30	DA, JC (BE, DE)	50S ribosomal protein L3	polymer	204	22250.0	2	UniProt (Q5SHN8) Pfam (PF00297)	Thermus thermophilus
31	EA, KC (BF, DF)	50S ribosomal protein L4	polymer	207	23014.7	2	UniProt (Q5SHN9) Pfam (PF00573)	Thermus thermophilus
32	FA, LC (BG, DG)	50S ribosomal protein L5	polymer	181	20930.4	2	UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673)	Thermus thermophilus
33	GA, MC (BH, DH)	50S ribosomal protein L6	polymer	159	17363.1	2	UniProt (Q5SHQ3)	Thermus thermophilus
34	HA, NC (BI, DI)	50S ribosomal protein L9	polymer	145	16093.9	2	UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948)	Thermus thermophilus
35	IA, OC (BJ, DJ)	50S ribosomal protein L10	polymer	130	9258.1	2		Thermus thermophilus
36	JA, PC (BN, DN)	50S ribosomal protein L13	polymer	138	15699.7	2	UniProt (P60488) Pfam (PF00572)	Thermus thermophilus
37	KA, QC (BO, DO)	50S ribosomal protein L14	polymer	122	13323.6	2	UniProt (Q5SHP8) Pfam (PF00238)	Thermus thermophilus
38	LA, RC (BP, DP)	50S ribosomal protein L15	polymer	146	15858.5	2	UniProt (Q5SHQ7) Pfam (PF00828)	Thermus thermophilus
39	MA, SC (BQ, DQ)	50S ribosomal protein L16	polymer	141	15993.9	2	UniProt (P60489) Pfam (PF00252)	Thermus thermophilus
40	NA, TC (BR, DR)	50S ribosomal protein L17	polymer	117	13619.0	2	UniProt (Q9Z9H5) Pfam (PF01196)	Thermus thermophilus
41	OA, UC (BS, DS)	50S ribosomal protein L18	polymer	98	10941.9	2	UniProt (Q5SHQ4) Pfam (PF00861)	Thermus thermophilus
42	PA, VC (BT, DT)	50S ribosomal protein L19	polymer	137	16218.8	2	UniProt (P60490) Pfam (PF01245)	Thermus thermophilus
43	QA, WC (BU, DU)	50S ribosomal protein L20	polymer	117	13572.0	2	UniProt (P60491) Pfam (PF00453)	Thermus thermophilus
44	RA, XC (BV, DV)	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (P60492) Pfam (PF00829)	Thermus thermophilus
45	SA, YC (BW, DW)	50S ribosomal protein L22	polymer	113	12750.0	2	UniProt (Q5SHP3) Pfam (PF00237)	Thermus thermophilus
46	TA, ZC (BX, DX)	50S ribosomal protein L23	polymer	92	10273.1	2	UniProt (Q5SHP0) Pfam (PF00276)	Thermus thermophilus
47	AD, UA (BY, DY)	50S ribosomal protein L24	polymer	100	11078.5	2	UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136)	Thermus thermophilus
48	BD, VA (BZ, DZ)	50S ribosomal protein L25	polymer	176	19892.9	2	UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693)	Thermus thermophilus
49	CD, WA (B0, D0)	50S ribosomal protein L27	polymer	84	9397.9	2	UniProt (P60493) Pfam (PF01016)	Thermus thermophilus
50	DD, XA (B1, D1)	50S ribosomal protein L28	polymer	93	10458.4	2	UniProt (P60494) Pfam (PF00830)	Thermus thermophilus
51	ED, YA (B2, D2)	50S ribosomal protein L29	polymer	71	8539.1	2	UniProt (Q5SHP6) Pfam (PF00831)	Thermus thermophilus
52	FD, ZA (B3, D3)	50S ribosomal protein L30	polymer	59	6670.0	2	UniProt (Q5SHQ6) Pfam (PF00327)	Thermus thermophilus
53	AB, GD (B4, D4)	50S ribosomal protein L31	polymer	30	3278.9	2	UniProt (Q5SJE1) Pfam (PF01197)	Thermus thermophilus
54	BB, HD (B5, D5)	50S ribosomal protein L32	polymer	59	6590.9	2	UniProt (P80339) Pfam (PF01783)	Thermus thermophilus
55	CB, ID (B6, D6)	50S ribosomal protein L33	polymer	44	5473.4	2	UniProt (P35871) Pfam (PF00471)	Thermus thermophilus
56	DB, JD (B7, D7)	50S ribosomal protein L34	polymer	48	5975.3	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus
57	EB, KD (B8, D8)	50S ribosomal protein L35	polymer	63	7245.9	2	UniProt (Q5SKU1) Pfam (PF01632)	Thermus thermophilus
58	FB, LD (B9, D9)	50S ribosomal protein L36	polymer	36	4304.2	2	UniProt (Q5SHR2) Pfam (PF00444)	Thermus thermophilus
59	EC (CX)	mRNA	polymer	4	1241.8	1		Synthetic construct
60	HC (DC)	50S Ribosomal protein L1	polymer	196	17103.1	1		Thermus thermophilus

Sequence modifications

AI, CI: 2 - 128 (UniProt: P80374)

PDB	External Database	Details
Arg 58	His 58	CONFLICT

AY, CY: 1 - 75 (GenBank: 443419838)

PDB	External Database	Details
-	C 2796112	DELETION

BA, DA: 0 - 2914 (GenBank: 55771382)

PDB	External Database	Details
A 1151	G 10391	CONFLICT

BU, DU: 2 - 118 (UniProt: P60491)

PDB	External Database	Details
Ala 32	Phe 32	CONFLICT

BW, DW: 1 - 113 (UniProt: Q5SHP3)

PDB	External Database	Details
Ala 113	Lys 113	CONFLICT

B1, D1: 3 - 95 (UniProt: P60494)

PDB	External Database	Details
Arg 81	Lys 81	CONFLICT

Contents of the asymmetric unit

Polymers	Number of chains	116
	Total formula weight	4454288.2
All*	Total formula weight	4454288.2

*Water molecules are not included.