4V90

Thermus thermophilus Ribosome

This is a non-PDB format compatible entry.

Replaces:  4BTC

Summary for 4V90

• Entry DOI: 10.2210/pdb4v90/pdb
• Descriptor: 16S RIBOSOMAL RNA, 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (62 entities in total)
• Functional Keywords: ribosome, translocation, gtpase activation rotation
• Biological source: THERMUS THERMOPHILUS HB8; More
• Total number of polymer chains: 58
• Total formula weight: 2320929.09

Authors

Chen, Y.,Feng, S.,Kumar, V.,Ero, R.,Gao, Y.G. (deposition date: 2014-02-22, release date: 2014-07-09, Last modification date: 2024-10-23)

Primary citation

Chen, Y.,Feng, S.,Kumar, V.,Ero, R.,Gao, Y.G.
Structure of EF-G-ribosome complex in a pretranslocation state.
Nat. Struct. Mol. Biol., 20:1077-1084, 2013

PubMed Abstract: In protein synthesis, elongation factor G (EF-G) facilitates movement of tRNA-mRNA by one codon, which is coupled to the ratchet-like rotation of the ribosome complex and is triggered by EF-G-mediated GTP hydrolysis. Here we report the structure of a pretranslocational ribosome bound to Thermus thermophilus EF-G trapped with a GTP analog. The positioning of the catalytic His87 into the active site coupled to hydrophobic-gate opening involves the 23S rRNA sarcin-ricin loop and domain III of EF-G and provides a structural basis for the GTPase activation of EF-G. Interactions of the hybrid peptidyl-site-exit-site tRNA with ribosomal elements, including the entire L1 stalk and proteins S13 and S19, shed light on how formation and stabilization of the hybrid tRNA is coupled to head swiveling and body rotation of the 30S as well as to closure of the L1 stalk.

PubMed: 23912278
DOI: 10.1038/nsmb.2645

Experimental method

X-RAY DIFFRACTION (2.95 Å)