4V7H

Structure of the 80S rRNA and proteins and P/E tRNA for eukaryotic ribosome based on cryo-EM map of Thermomyces lanuginosus ribosome at 8.9A resolution

This is a non-PDB format compatible entry.

Summary for 4V7H

• Entry DOI: 10.2210/pdb4v7h/pdb
• EMDB information: 1345
• Descriptor: 18S rRNA, 40S ribosomal protein S14(A), 40S ribosomal protein S23(A), ... (53 entities in total)
• Functional Keywords: eukaryotic ribosome, 80s, rack1 protein, flexible fitting, ribosome
• Biological source: Thermomyces lanuginosus; More
• Total number of polymer chains: 53
• Total formula weight: 2462331.63

Authors

Taylor, D.J.,Devkota, B.,Huang, A.D.,Topf, M.,Narayanan, E.,Sali, A.,Harvey, S.C.,Frank, J. (deposition date: 2009-09-22, release date: 2014-07-09, Last modification date: 2024-02-28)

Primary citation

Taylor, D.J.,Devkota, B.,Huang, A.D.,Topf, M.,Narayanan, E.,Sali, A.,Harvey, S.C.,Frank, J.
Comprehensive molecular structure of the eukaryotic ribosome.
Structure, 17:1591-1604, 2009

PubMed Abstract: Despite the emergence of a large number of X-ray crystallographic models of the bacterial 70S ribosome over the past decade, an accurate atomic model of the eukaryotic 80S ribosome is still not available. Eukaryotic ribosomes possess more ribosomal proteins and ribosomal RNA than do bacterial ribosomes, which are implicated in extraribosomal functions in the eukaryotic cells. By combining cryo-EM with RNA and protein homology modeling, we obtained an atomic model of the yeast 80S ribosome complete with all ribosomal RNA expansion segments and all ribosomal proteins for which a structural homolog can be identified. Mutation or deletion of 80S ribosomal proteins can abrogate maturation of the ribosome, leading to several human diseases. We have localized one such protein unique to eukaryotes, rpS19e, whose mutations are associated with Diamond-Blackfan anemia in humans. Additionally, we characterize crucial interactions between the dynamic stalk base of the ribosome with eukaryotic elongation factor 2.

PubMed: 20004163
DOI: 10.1016/j.str.2009.09.015

Experimental method

ELECTRON MICROSCOPY (8.9 Å)