4V6G

Initiation complex of 70S ribosome with two tRNAs and mRNA.

This is a non-PDB format compatible entry.

Summary for 4V6G

• Entry DOI: 10.2210/pdb4v6g/pdb
• Related: 3I8F 3I8G 3I8H 3I8I 3I9C 3I9D 3I9E
• Descriptor: 16S RRNA (E.COLI NUMBERING), 30S RIBOSOMAL PROTEIN S10, 30S RIBOSOMAL PROTEIN S11, ... (55 entities in total)
• Functional Keywords: ribosome, trna, mrna
• Biological source: Thermus thermophilus; More
• Total number of polymer chains: 109
• Total formula weight: 4520213.78

Authors

Jenner, L.B.,Yusupova, G.,Yusupov, M. (deposition date: 2009-07-10, release date: 2014-07-09, Last modification date: 2024-12-25)

Primary citation

Jenner, L.B.,Demeshkina, N.,Yusupova, G.,Yusupov, M.
Structural aspects of messenger RNA reading frame maintenance by the ribosome.
Nat.Struct.Mol.Biol., 17:555-560, 2010

PubMed Abstract: One key question in protein biosynthesis is how the ribosome couples mRNA and tRNA movements to prevent disruption of weak codon-anticodon interactions and loss of the translational reading frame during translocation. Here we report the complete path of mRNA on the 70S ribosome at the atomic level (3.1-A resolution), and we show that one of the conformational rearrangements that occurs upon transition from initiation to elongation is a narrowing of the downstream mRNA tunnel. This rearrangement triggers formation of a network of interactions between the mRNA downstream of the A-site codon and the elongating ribosome. Our data elucidate the mechanism by which hypermodified nucleoside 2-methylthio-N6 isopentenyl adenosine at position 37 (ms(2)i(6)A37) in tRNA(Phe)(GAA) stabilizes mRNA-tRNA interactions in all three tRNA binding sites. Another network of contacts is formed between this tRNA modification and ribosomal elements surrounding the mRNA E/P kink, resulting in the anchoring of P-site tRNA. These data allow rationalization of how modification deficiencies of ms(2)i(6)A37 in tRNAs may lead to shifts of the translational reading frame.

PubMed: 20400952
DOI: 10.1038/nsmb.1790

Experimental method

X-RAY DIFFRACTION (3.5 Å)